Nucleotide Sequence of the Capsid Protein Gene of Plum Pox Potyvirus

Nucleotide Sequence of the Capsid Protein Gene of Plum Pox Potyvirus

The sequence of 1232 nucleotides from the 3' region of the plum pox virus (PPV) RNA has been determined. This sequence contains one long open reading frame (ORF). The sequence of the 17 amino-terminal amino acids of the PPV capsid protein was determined chemically. An identical amino acid sequence was found in the translation product of the large ORF, starting at amino acid 330, which is alanin...

Nucleotide sequence of the 3'-terminal region of the RNA of the El Amar strain of plum pox potyvirus.

The nucleotide sequence of the 3'-terminal 4773 nucleotides of the RNA of a widely divergent, aphid-transmissible strain of plum pox potyvirus isolated from Egypt (PPV-El Amar) was determined. The sequenced region covers the carboxy terminus of the cylindrical inclusion (CI) gene, and the putative 6K protein, the NIa protease, the NIb RNA polymerase and the coat protein genes, linked together a...

Nucleotide Sequence of Gene Encoding Capsid Protein VPI of Foot-and-Mouth Disease Virus / Type O1 Iran

nucleotide sequence of gene encoding capsid protein vpi of foot-and-mouth disease virus / type o1 iran

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A highly sensitive immunocapture polymerase chain reaction method for plum pox potyvirus detection.

A highly sensitive assay, based on polymerase chain reaction amplification of cDNA synthesized from the viral RNA of antibody-captured viral particles, has been developed for plum pox potyvirus (PPV) detection. The reaction, called immunocapture/PCR (IC/PCR), yields a specific 243-bp product. The immunocapture step, by allowing the use of large sample volumes and by the viral particle prepurifi...